A analysis group from Seoul Nationwide College of Science and Expertise, Republic of Korea, led by Professor Insup Noh, has developed a bioink utilizing nanocellulose derived from Kombucha SCOBY (Symbiotic Tradition of Micro organism and Yeast) as a scaffold materials. The research was printed within the Worldwide Journal of Organic Macromolecules.
Tissue engineering facilitates the regeneration of broken tissues, together with pores and skin, cartilage, and organs, by rising human cells on scaffolds utilizing 3D printing and bioink. The developed biomaterial is suitable with a conveyable “Biowork” biopen, additionally designed by the analysis group, providing an alternative choice to typical scaffold supplies.
The digital biopen permits for the exact software of bioink to irregular surfaces, resembling cartilage defects and intensive pores and skin wounds. This strategy allows direct in vivo tissue restore, decreasing the necessity for in vitro tissue engineering processes.
Our prefabricated nanocellulose hydrogel community from symbiotic tradition of micro organism and yeast has the potential for use as a platform bioink for in vivo tissue engineering by loading all varieties of biomolecules and medicines and direct bioprinting.
Insup Noh, Professor, Seoul Nationwide College of Science and Expertise
Kombucha SCOBY, a symbiotic tradition of yeast and micro organism, ferments inexperienced tea and produces biocompatible and biodegradable nanocellulose. Nevertheless, the entangled construction of Kombucha SCOBY-derived nanocellulose requires modification for 3D bioprinting. Changes to its rheological (circulate) and mechanical properties are crucial to enhance extrusion and keep structural integrity after printing.
To reinforce bioprintability, researchers partially hydrolyzed nanocellulose utilizing acetic acid, breaking glucose bonds and decreasing community entanglement. Nevertheless, this therapy weakened its structural power attributable to a scarcity of property management. To bolster the fabric, the group included negatively charged kaolin and positively charged chitosan nanoparticles. These parts work together electrostatically with cellulose to kind a secure hydrogel appropriate for 3D bioprinting.
The bioink was ready by mixing all parts, together with reside cells, straight inside a biopen. The biopen, managed by a pc, contained two counter-rotating screws that uniformly mixed the substances, permitting direct software to broken tissue by way of a needle.
When related to a 3D bioprinter, the biopen produced high-resolution, multilayer, self-supporting constructions, resembling bifurcated tubes and pyramids over 1 cm in peak. It was additionally used for direct in situ layer-by-layer printing to fill irregularly formed defects. The researchers efficiently utilized the bioink to exactly fill 3D-printed femoral head and skull molds with predefined defects.
The mix of bioink and a digital biopen gives a cheap methodology for treating massive or irregularly formed wounds, notably in emergency and first-aid functions, with out requiring an in vitro tissue regeneration course of.
This expertise permits for a fast and simple one-step course of the place the drug and hydrogel are blended and instantly utilized on-site to injured areas of various shapes.
Insup Noh, Professor, Seoul Nationwide College of Science and Expertise
Journal Reference:
Bhattacharyya, A., et al. (2025) Simultaneous processing of each handheld biomixing and biowriting of kombucha cultured pre-crosslinked nanocellulose bioink for regeneration of irregular and multi-layered tissue defects. Worldwide Journal of Organic Macromolecules. doi.org/10.1016/j.ijbiomac.2024.136966
