Researchers from the Institute for Molecular Science aimed to stage out the velocity distinction between synthetic motors and motor proteins by bettering the nanoscale synthetic motor utilizing their understanding of molecular motors. The research was printed in Nature Communications.
Picture Credit score: Takanori Harashima
DNA-nanoparticle motors are minuscule synthetic motors that use RNA and DNA constructions to drive movement via enzymatic RNA degradation. The Brownian movement is biased to remodel chemical power into mechanical movement.
The DNA-nanoparticle motor employs the “burnt-bridge” Brownian ratchet mechanism. The degradation (or “burning”) of the bonds (or “bridges”) that the motor crosses alongside the substrate propels this type of motion, successfully biasing the motor’s movement ahead.
These extremely programmable nano-sized motors could be made for transport, diagnostics, and molecular computation functions. The issue is that DNA-nanoparticle motors are usually not as quick as their organic counterparts, the motor protein, regardless of their genius. Researchers use geometry-based kinetic simulation and single-particle monitoring experiments to investigate, optimize, and rebuild a quicker synthetic motor.
Pure motor proteins play important roles in organic processes, with a velocity of 10-1000 nm/s. Till now, synthetic molecular motors have struggled to method these speeds, with most typical designs reaching lower than 1 nm/s.
Takanori Harashima, Researcher and Examine First Writer, Institute for Molecular Science
Switching the bottleneck is a steered answer to the velocity downside. The experiment and simulation demonstrated that the binding of RNase H serves because the bottleneck, slowing down your complete course of.
RNase H breaks down RNA in RNA/DNA hybrids within the motor and is concerned in genome upkeep. A slower complete processing time outcomes from longer pauses in movement attributable to slower RNase H binding. The velocity was considerably enhanced by rising the RNase H focus, decreasing pause durations from 70 s to about 0.2 s.
Nonetheless, run size (the space the motor travels earlier than detaching) and processivity (the variety of steps earlier than detachment) had been sacrificed to extend the motor velocity. In line with the researchers, the next DNA/RNA hybridization charge might improve this trade-off between velocity and processivity/run size, bringing the simulated efficiency nearer to that of a motor protein.
The engineered motor achieved a velocity of 30 nm/s, 200 processivity, and a 3 μm run-length with redesigned DNA/RNA sequences and a 3.8-fold enhance in hybridization charge. The research reveals that the DNA-nanoparticle motor can now perform equally to a motor protein.
Finally, we purpose to develop synthetic molecular motors that surpass pure motor proteins in efficiency.
Takanori Harashima, Researcher and Examine First Writer, Institute for Molecular Science
These synthetic motors could be extremely useful in molecular computations based mostly on the motor’s movement and their potential for extremely delicate prognosis of infections or disease-related molecules.
The simulation and experiment carried out on this research supply a promising future for DNA nanoparticles and associated synthetic motors, their capability to imitate motor proteins, and their makes use of in nanotechnology.
Researchers Ryota Iino, Akihiro Otomo, and Takanori Harashima from the Graduate Institute for Superior Research at SOKENDAI and the Institute for Molecular Science on the Nationwide Institutes of Pure Sciences participated on this research.
This research was funded by the Tsugawa Basis Analysis Grant for FY2023, JST ACT-X “Life and Info”, Grant-in-Support for Transformative Analysis Areas (A) (Publicly Provided Analysis) “Supplies Science of Meso-Hierarchy” and “Molecular Cybernetics”, Grant-in-Support for Scientific Analysis on Revolutionary Areas “Molecular Engine”, JST ACT-X “Life and Info”, and JSPS KAKENHI.
Journal Reference:
Harashima, T., et al. (2025) Rational engineering of DNA-nanoparticle motor with excessive velocity and processivity akin to motor proteins. Nature Communications. doi.org/10.1038/s41467-025-56036-0
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